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. Author manuscript; available in PMC: 2019 Oct 23.
Published in final edited form as: ACS Nano. 2018 Aug 23;12(10):10130–10141. doi: 10.1021/acsnano.8b04947

Figure 3. Dynamic topographical structure of PEG shells significantly reduced the uptake of PEGylated NPs by endothelial cells in the liver.

Figure 3.

Liver cells are stained with CD146 and CD68. (a) Representative histogram of NP internalization of all cells in the livers collected from mice 4 h after tail vein injection of saline, PLGA-PEG-NP or PLGA-TPEG-NP-20. (b) Percentage of cells that internalized NPs (DiD+) within hepatocytes, endothelial cells, Kupffer cells and other cells. (c-f) Representative histograms of NP internalization of endothelial cells, Kupffer cells, hepatocytes, and other cells in the livers. (g) Relative mean fluorescence intensity (MFI) of different liver cells. (h) Relative MFI of hepatocytes that internalized NPs or not (NP+/NP−). (i) Relative MFI of other cells that internalized NPs or not (NP+/NP−). Values indicate mean ± SD (n = 5 from three independent experiments). *, P < 0.05, ***, P < 0.001.