Evaluation of RNase T1 as a potential enzyme for the characterization of single stranded and double stranded miRNA: (a) ss-Cy5-antimiR-21 measured for fluorescence signal before and after RNase T1 treatment, and after separation of rGO bound and rGO free fractions from RNase T1 treated samples, (b) ds-Cy5-antimiR-21 measured for fluorescence signal before and after RNase T1 treatment, and after separation of rGO bound and rGO free fractions from RNase T1 treated samples, (c) ss-Cy5-antimiR-21 measured for fluorescence signal by treatment with RNase T1 and mixed with rGO, and the rGO bound and rGO free fractions further treated with RNase T1 of the miRNAs of different forms present in the fractions, and (d) ds-Cy5-antimiR-21 measured for fluorescence signal by treatment with RNase T1 and mixed with rGO, and the rGO bound and rGO free fractions further treated with RNase T1 for the miRNAs of different forms present in the fractions.