Figure 4.

miR-339 directly targets ZNF689 expression.

Notes: (A) miR-339 and its presumptive binding sites in the 3′-UTR of ZNF689. Mutations were generated in the complementary site which binds to the seed region of miR-339. (B) miR-339 mimic and luciferase plasmids which include WT ZNF689 3′-UTR or Mut ZNF689-3′-UTR were co-transfected into HCCLM3 cells. The relative luciferase activity was determined. (C) The mRNA level of ZNF689 was measured by using qPCR after miR-339 mimic was transfected into HCCLM3 cells. (D) The protein expression of ZNF689 was detected by using Western blotting after miR-339 mimic was transfected into HCCLM3 cells. (E) Data represent the relative protein expression, shown as mean ± SD, n=3. *P<0.05, **P<0.01 vs the scramble group.

Abbreviations: Mut, mutant type; qPCR, quantitative PCR; WT, wild type.