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. Author manuscript; available in PMC: 2019 Jan 28.
Published in final edited form as: Cell Rep. 2018 Oct 9;25(2):383–397.e10. doi: 10.1016/j.celrep.2018.09.034

Figure 2. AIF Deficiency in POMC Neurons Enhances Leptin Sensitivity in Obesity.

Figure 2.

(A–C) Absolute (A) and percentage of (B) 3-day food intake and body-weight change (C) of HFD-fed obese AIFΔPOMC and AIFfl control mice after 3 days of twice daily intraperitoneal injection of leptin (2 mg/kg) versus vehicle (0.1 M PBS) (n = 7–9 per group).

(D and E) Representative confocal images (D) and quantification (E) of pSTAT3-immunoreactive POMC neurons (pSTAT3, green; POMC, red) and corresponding nuclear counterstaining (DAPI, blue) in the ARH of 16-hr-fasted HFD-fed AIFΔPOMC and AIFfl control mice 45 min after i.p. injection of leptin (2 mg/kg) versus vehicle (0.1 M PBS) (n = 3–6 per group).

Scale bars, 100 μm; 3V, third ventricle. Data are represented as mean ± SEM. *p ≤ 0.05 and **p < 0.01 as determined by two-way ANOVA followed by Bonferroni post hoc test. See also Figure S3.