KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-AIF | Abcam | Cat# ab32516; RRID: AB_726995 |
| Anti-NDUFA9 | Invitrogen | Cat# 459100; RRID: AB_2532223 |
| Anti-NDUFB8 | Invitrogen | Cat# 459210; RRID:AB_2532232 |
| Anti-Complex II 70 kDa Fp Subunit | Invitrogen | Cat# 459200; RRID: AB_2532231 |
| Anti-UQCRC1 | Invitrogen | Cat# 459140; RRID: AB_2532227 |
| Anti-Complex IV | Invitrogen | Cat# 459110; RRID: AB_2532224 |
| Anti-ATP synthase subunit β | Invitrogen | Cat# 21351; RRID: AB_221512 |
| Anti-β-actin | Sigma Aldrich | Cat# A2228; RRID: AB_476697 |
| Anti-calnexin | Merck Millipore | Cat# 208880; RRID: AB_2069031 |
| Anti-phospho-STAT3 (Tyr705) | Cell Signaling | Cat# 9145; RRID: AB_2491009 |
| Anti-POMC antibody | Phoenix | Cat# H029–30; RRID: AB_2037442 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Insulin | Sanofi Aventis, Germany | Insuman rapid |
| Mouse Leptin | Sigma Aldrich | Cat# L3772–1MG |
| Honokiol | Sigma Aldrich | Cat# H4914–10MG |
| Seahorse XF Palmitate-BSA FAO Substrate | Agilent Technologies | Cat# 102720–100 |
| MitoSox Red Mitochondrial Superoxide Indicator | Invitrogen | Cat# M36008 |
| MitoTracker Green FM | Invitrogen | Cat# M7514 |
| TSA® Plus Cyanine 3 | Perkin Elmer | Cat# NEL744E001KT |
| TSA® Plus Cyanine 5 | Perkin Elmer | Cat# NEL745E001KT |
| Dihydroethidium (DHE) | Invitrogen | Cat# D1168 |
| Critical Commercial Assays | ||
| Arcturus picopure RNA isolation Kit | Thermo Fisher Scientific | Cat# KIT0204 |
| Mouse ultra-sensitivity insulin ELISA | Crystal Chem, USA | Cat# 90080 |
| Mouse Leptin ELISA | Crystal Chem, USA | Cat# 90030 |
| Mouse Corticosterone ELISA | Enzo Life Sciences, USA | Cat# ADI-900–097 |
| Dental acrylic Super Bond C&B | Sun Medical | Cat# 7100 |
| CyQUANT® Cell Proliferation Assay Kit | ThermoFisher Scientific | Cat# C7026 |
| XF Cell Mito Stress Test Kit | Agilent Technologies | Cat# 101706–100 |
| TSA Plus Fluorescence kit | Perkin Elmer, USA | Cat# NEL741001KT |
| RNAscope Enhancer Fluorescent Kit v2 | ACD | Cat# 323100 |
| Experimental Models: Cell Lines | ||
| Embryonic Mouse Hypothalamus Cell Line N43/5 | (Choi et al., 2010) | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: Pomc-Cre | (Balthasar et al., 2004) | N/A |
| Mouse: AIF-flox | (Joza et al., 2005) | N/A |
| Mouse: ROSA26-flox-LacZ | (Plum et al., 2006) | N/A |
| Mouse: POMC-EGFP | (Cowley et al., 2001) | N/A |
| Mouse: MPC1 | (Gray et al., 2015) | N/A |
| Oligonucleotides | ||
| Taqman probe AIFm1 | Thermo Fisher | Mm00442545_m1 |
| Taqman probe CPT1b | Thermo Fisher | Mm00487191_g1 |
| Taqman probe PPARg | Thermo Fisher | Mm00440945_m1 |
| mitochondrial Nd2: fw: 5′- AGGGATCCCACTGCACATAG-3′; | Eurogentec | https://secure.eurogentec.com/life-science.html |
| mitochondrial Nd2: rev: 5′- CTCCTCATGCCCCTATGAAA-3′ | Eurogentec | https://secure.eurogentec.com/life-science.html |
| mitochondrial D-Loop: fw: 5′- GGTTCTTACTTCAGGGCCATCA-3′ | Eurogentec | https://secure.eurogentec.com/life-science.html |
| mitochondrial D-Loop: rev: 5′-GATTAGACCCGATACCATCGAGAT-3′ | Eurogentec | https://secure.eurogentec.com/life-science.html |
| nuclear Nduv: fw: 5′- CTTCCCCACTGGCCTCAAG-3′; | Eurogentec | https://secure.eurogentec.com/life-science.html |
| nuclear Nduv: rev: 5′- CCAAAACCCAGTGATCCAGC-3′ | Eurogentec | https://secure.eurogentec.com/life-science.html |
| Aifm1 antisense LNA: 5′-TAGCGGAGGCAATGGC-3′ | Exiquon | Cat# 300600, design-ID: 657181–3 |
| Negative control A antisense LNA: 5′-AACACGTCTATACGC-3′ | Exiquon | 300610 |
| Custom-designed RNA scope probe targeting exons 7–8 of the AIFm1 transcript (target region: 901–1081) | ACD | Cat# NM_012019.3 |
| Custom-designed RNA scope probe targeting exons 3–5 of the MPC1 transcript (target region: 155–885) | ACD | Cat# NM_018819.4 |
| RNA scope tdTomato probe (target region: 7 – 1382) | ACD | Cat# 317041 |
| RNA scope POMC probe (target region 19–995) | ACD | Cat# NM_008895.3 |
| Software and Algorithms | ||
| GraphPad Prism 7 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| Fiji (ImageJ) Software Package (incl. Adiposoft Plugin) | (Schneider et al., 2012) | http://imagej.net/Adiposoft |
| IVIS LivingImage Software V4.3.1 | Caliper LifeScience, Perkin Elmer, USA | http://www.perkinelmer.com:80/category/in-vivo-imaging-software |
| Vinci software package 4.61.0 | (Cízek et al., 2004) | http://www.nf.mpg.de/vinci3 |
| FlowJo (Ver. 10.0.8r1) | Treestar | https://www.flowjo.com/ |
| PatchMaster (version 2.32) | HEKA, Lambrecht, Germany | https://www.heka.com/downloads/downloads_main.html |
| Patcher’s Power Tools plug-in | Wavemetrics, Lake Oswego, OR, USA | httpx://www.mpibpc.mpg.de/groups/neher/index.php?page=software |
| Spike2 (version 7) | Cambridge Electronic Design Ltd., Cambridge, UK | http://ced.co.uk/de/ |
| Other | ||
| Regular Chow Diet | sniff Spezialdiäten GmbH, Germany | R/M-H Phytoestrogenarm |
| Regular Chow Diet (Teklad) | Envigo, USA | Cat#2918 |
| High Fat Diet | sniff Spezialdiäten GmbH, Germany | Cat# D12492-(I) mod. |
| Normal Control Diet | sniff Spezialdiäten GmbH, Germany | Cat# D12450-(B) mod LS |
| High Fat Diet (Altromin) | Altromin Spezialfutter GmbH & Co. KG, Germany | Cat# C1057 |
| NMR Analyzer minispeq mq7.5 | Bruker Optik, Ettlingen, Germany | https://www.bruker.com/ |
| IVIS Spectrum CT scanner | Caliper LifeScience, Perkin Elmer, USA | Cat# 128201 |
| Indirect calorimetry system “PhenoMaster” | TSE systems, Chesterfield, USA | http://www.tse-systems.com/product-details/phenomaster/ |
| Lipovenös® MCT 20% | FRESENIUS KABI Germany | PZN:276848 |
| XFe96 Extracellular Flux Analyzer, “Seahorse” | Agilent Technologies | https://www.agilent.com/en/products/cell-analysis/seahorse-analyzers/seahorse-xfe96-analyzer |
| MACSQuant VYB | Miltenyi Biotec | https://www.miltenyibiotec.com/DE-en/ |
| Vibration microtome HM-650 V | Thermo Scientific, Walldorf, Germany | https://uk.vwr.com/store/product/13046604/vibrating-microtome-hm-650-v |
| Inline solution heater | Warner Instruments, Hamden, CT, USA | Cat# SH27B |
| Temperature controller | Warner Instruments, Hamden, CT, USA | Cat# TC-324B |
| Electrodes | Science Products | Cat# GB150–8P |
| Vertical pipette puller | Narishige, London, UK | Cat# PP-830 |
| EPC10 patch-clamp amplifier | HEKA, Lambrecht, Germany | https://www.heka.com/ |