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. 2019 Jan 4;10(2):133–151. doi: 10.18632/oncotarget.26512

Figure 2. Reciprocal expression of AnxA6 and RasGRF2 in TNBC cells.

Figure 2

(A, D and G) Representative AnxA6-expressing BT-549 and the AnxA6-low HCC1806 (A), control and AnxA6 down-regulated BT-549 (D) or control and AnxA6 up-regulated HCC1806 (G) breast cancer cell lines were grown to 80% confluency then harvested. Equal amounts of whole cell extracts were analyzed by Western blotting using antibodies against AnxA6, RasGRF2 or β-actin. (B, E, H) Densitometric analysis of AnxA6 and RasGRF2 expression in parental BT-549 and HCC1806 cells (B), control and AnxA6 down-regulated BT-549 (E) or control and AnxA6 up-regulated HCC1806 (H) breast cancer cell lines. Bars represent β-actin normalized AnxA6 and RasGRF2 expression from three independent experiments (B and H) or from a representative experiment (E). (C, F, I) Analysis of the relationship between AnxA6 and RasGRF2 mRNA expression in TNBC cells (C), mesenchymal-like TNBC cells (F) and basal-like TNBC cells (I). The mesenchymal-like BT-549 is represented by the red dot, while the basal-like HCC1806 and MDA-MB-468 are represented by the green and yellow dots respectively. TNBC: triple negative breast cancer; MSL BC: mesenchymal-like breast cancer; BLBC: basal-like breast cancer.