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. Author manuscript; available in PMC: 2020 Feb 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2019 Jan 3;365:61–70. doi: 10.1016/j.taap.2019.01.003

Figure 1. Determination of neutral lipid accumulation by ORO staining in OA-loaded HepaRG cells.

Figure 1.

HepaRG cells were incubated for 48 hours in HepaRG treatment medium alone (CON) or containing 0.7% FF-BSA, either alone or complexed with 0.33 mM, 0.66 mM, or 1 mM OA. Treatment medium was replaced once after 24 hours. (A) ORO was used to stain neutral lipids, and cells were photographed under phase-contrast optics at 200X magnification. Neutral lipids are stained red, hepatocyte nuclei are purple/blue. (B) ORO was extracted from the cells and absorbance measured at 510 nm. Each bar represents the mean ± SEM (n=4 wells per group, derived from combining the data from 2 independent HepaRG experiments with duplicate treatments). *Significantly different from vehicle control (FF-BSA), P<0.05, **P<0.01.