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. Author manuscript; available in PMC: 2020 Feb 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2019 Jan 3;365:61–70. doi: 10.1016/j.taap.2019.01.003

Figure 3. Effects of FOH on nuclear receptor (CAR, PPARα, and FXR) activities in HepaRG cells.

Figure 3.

HepaRG cells were cultured in HepaRG treatment medium containing FF-BSA + 0.1% ethanol (EtOH), FF-BSA + 100 μM FOH, 0.1% DMSO, or one of the following nuclear receptor agonists: 0.1 μM CITCO (CAR), 10 μM GW7647 (PPARα), or 1 μM GW4064 (FXR). Treatment medium was replaced once after 24 hours. After 48 hours of treatment, cells were harvested for RNA isolation, and CYP2B6, PLIN2, and SHP mRNA levels were quantified by RT-qPCR, as described in Materials and Methods. Each bar represents the mean ± SEM of normalized mRNA values from 3 (PLIN2, SHP; n=3) or 4 (CYP2B6; n=4) independent experiments, and within each experiment data were normalized to the EtOH-treated control group (i.e., EtOH=1). *Significantly different from 1, P<0.05.#Significantly different from DMSO-treated group, P<0.05.