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Orbitrap LCMS traces of underivatized OT in serum from non-pregnant/non-lactating/non-medicated women without spiking (A) and after spiking with 50 pg/mL OT (B) and applying a 3–5 ppm mass window when monitoring in targeted SIM mode. Differences in retention times are probably due to slight differences in mobile phase composition during gradient elution.
