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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Am J Transplant. 2018 Aug 24;19(2):356–367. doi: 10.1111/ajt.15043

Figure 7. Neutrophil HO-1 deficiency enhances reactive oxygen species (ROS) production and inflammatory gene phenotype.

Figure 7

Casein-elicited peritoneal neutrophils were obtained from HO-1 proficient control and myeloid-specific HO-1 knockout (mHO-1 KO) mice. (A) ROS level in neutrophil was analyzed by flow cytometry using fluorescent CellROX probe (n=4/group). (B) qRT-PCR-assisted detection of mRNA coding for HO-1, TNFα, CXCL1, CXCL2 and CXCL10. Data were normalized to β-actin gene (n=4/group). Data shown as mean±SD (*p<0.05, Student t-test).