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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Neurobiol Aging. 2018 Nov 8;75:11–24. doi: 10.1016/j.neurobiolaging.2018.10.027

Figure 2. Cortical tau hyperphosphorylation following acute midazolam administration persists up to 24h in C57BL/6 mice.

Figure 2.

Cortical tau phosphorylation (% Ctl) at the AT8 (A), CP13 (B), PHF-1 (C), 12E8 (D), AT180 (E), AT270 (F) and pS199 (G) phosphoepitopes in 8-10-week old C57BL/6 mice treated with midazolam (Mid) 25 mg/kg i.p and sacrificed 30 min (0.5h, n = 6) or 24h (n = 6) later. Control mice (n = 6) were treated with 0.9% saline i.p. and sacrificed 30 min later. Normothermia was maintained throughout and rectal temperatures at the end of the study were 37.4 ± 0.2°C (Ctl), 37.0 ± 0.3°C (Mid 0.5h), and 37.0 ± 0.4°C (Mid 24h). Phosphorylated tau levels were normalized to total tau (H) after controlling for gel loading with β-actin. Data are expressed as mean ± SD and **,***, **** denote P < 0.01, P <0.001, and P < 0.0001 vs. Ctl., respectively using ANOVA with Tukey’s post-hoc test. Two representative immunoblot bands are displayed for each condition.