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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Neurobiol Aging. 2018 Nov 8;75:11–24. doi: 10.1016/j.neurobiolaging.2018.10.027

Figure 4. The effect of acute midazolam administration on phosphatase levels in the mouse cortex at 0.5h and 24h.

Figure 4.

Cortical proteins from the in 8-10 week old C57BL/6 mice that received either saline (Ctl) or midazolam 25 mg/kg i.p. and were sacrificed 30 min (Mid 0.5) or 24h (Mid 24) were separated by SDS-PAGE and protein levels of several protein phosphatases were determined using antibodies directed at (A) the demethylated protein phosphatase catalytic subunit (dm-PP2A-C), (B) the PP2A catalytic subunit (PP2A-C), (C) protein phosphatase 1 (PP1), (D) protein phosphatase 2B (PP2B), and (E) protein phosphatase 5 (PP5). Relative immunoreactive band intensities are expressed as a percent of saline control (Ctl) after appropriately normalizing for gel loading with β-actin or β-tubulin. For each condition, 2 representative immunoblot bands are displayed with Ctl (n = 6), 0.5h (n = 6), and 24h (n = 6). Data are expressed as mean ± SD. * denotes P < 0.05 vs. Ctl using ANOVA with Tukey’s post hoc test.