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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Neurobiol Aging. 2018 Nov 8;75:11–24. doi: 10.1016/j.neurobiolaging.2018.10.027

Figure 8. Hippocampal tau phosphorylation following chronic midazolam administration persists as a function of age in young C57BL/6 mice.

Figure 8.

Hippocampal tau phosphorylation (% Ctl) at the AT8, CP13, PHF-1 phosphoepitopes in 8-10-week-old (A-C) C57BL/6 mice treated with a subcutaneous infusion of midazolam 3 mg/kg/h (n = 5) or 0.9% saline (Ctl; n = 6) and sacrificed 1 week after the start of the 24h infusion. Normothermia was maintained throughout the administration of midazolam or saline. Phosphorylated tau levels were normalized to total tau (D), after controlling for gel loading with β-actin. Relative immunoreactive band intensities are expressed as a percent of Ctl and are displayed for each phosphoepitope and total tau. For each condition, 2 representative immunoblot bands are displayed. Data are expressed as mean ± SD and *, *** denote P < 0.05 and P < 0.001, respectively, vs. Ctl using an unpaired t-test.