Table 2.
Reference set of melting temperatures (Tm°C) generated using the four molecular beacons following amplification of DNA from standard fungal strains. Beacon PFC11 gave a TM with all strains tested while PFA21 and PYCT41 gave adequate difference in Tm to enable speciation. Beacon PFM3 gave Tm with only zygomycetes.
| ATCC no. | Fungal strains | Molecular beacon |
|||
|---|---|---|---|---|---|
| PFC11 | PFA21 | PFM3 | PYCT41 | ||
| ATCC 204304 | Aspergillus flavus | 64.26 | 70.38 | – | 82.23 |
| ATCC 293 | Aspergillus fumigatus | 69.59 | 74 | – | 64.93 |
| ATCC 9508 | Aspergillus niger | 64.17 | 69.92 | – | 64.93 |
| Clinical isolate | Aspergillus terreus | 64.12 | 70.47 | – | 82.8 |
| Clinical isolate | Aspergillus nidulans | 64.21 | 70.34 | – | 81.96 |
| Clinical isolate | Aspergillus glaucus | 63.75 | 70.2 | – | 46 |
| Clinical isolate | Mucor | 49.66 | 46.44 | 59.03 | 61.72 |
| Clinical isolate | Rhizopus | 63.9 | 46.61 | 58.59 | 61.49 |
| ATCC 90028 | Candida albicans | 65.19 | 70.34 | – | 45.63 |
| ATCC 750 | Candida tropicalis | 65.19 | 70.31 | – | 62.47 |
| ATCC 6258 | Candida krusei | 65.33 | 70.38 | – | – |
| ATCC 22019 | Candida parapsilosis | 65.23 | 70.31 | – | 41.19 |
| ATCC 6258 | Candida guillermondii | 64.75 | 70.25 | – | – |
| ATCC 208821 | Cryptococcus neoformans | 62 | 68.91 | – | 61 |
| Clinical isolate | Trichosporon asahii | 61.09 | 69.04 | – | 61.72 |
| Clinical isolate | Penicillium spp. | 68.5 | 73.17 | – | – |
| Clinical isolate | Fusarium spp. | 55.1 | 57.22 | – | – |
| Clinical isolate | Microsporum gypseum | 68.35 | 73.08 | – | – |
| Clinical isolate | Epidermophyton mentagrophytes | 63.2 | – | – | – |
| Clinical isolate | Trichophyton rubrum | 61.58 | – | – | – |