Figure 2.

Pretreatment with low-dose Poly(I:C) reduces GILZ expression paralleled by sensitization toward LPS and Pam₃CSK₄. (A,B) AMs were left untreated (Co) or treated with LPS (100 ng/mL) or Poly(I:C) (PIC, 1 μg/mL) for 24 h. GILZ levels were determined by Western blot using tubulin as a loading control. (A) Representative blot. (B) GILZ signal intensities were quantified and normalized to tubulin values (n = 3, duplicates; ^**p < 0.01 compared with Co). (C,D) AMs were either left untreated (NT) or pretreated with PIC (1 μg/mL), followed by stimulation with LPS (1 μg/mL, C) or Pam₃CSK₄ (Pam, 1 μg/mL, D) for another 2 h. TNF, IL6, and CXCL8 mRNA expression was determined by qRT-PCR using ACTB as a housekeeping gene (n = 4, duplicates; ^**p < 0.01, ^***p < 0.001).