Table 2.
Primer | Sequence ( 5′ → 3′)a | Reference |
---|---|---|
TUtestF-BglII | TACGCAGATCTACTACTGTGGCTAAGCCTCAAC | This study |
TUtestR-XhoI | TACGCCTCGAGACGGAGAGTAACAACACGATGC | This study |
R6K-BglII | TACGCAGATCTCCATGTCAGCCGTTAAGTGT | This study |
R6K-XhoI | TACGCCTCGAGGTTGATCGGCACGTAAGAGG | This study |
R6K-BamHI | TACGCGGATCCGTTGATCGGCACGTAAGAGG | This study |
R6K-EcoRI | TACGCGAATTCCCATGTCAGCCGTTAAGTGT | This study |
P1 | GGCTGCAGACGCACAGCA | This study |
IR-F | TTTTTTGAAGTAAACTTCATAAGGTGTTTTCCAACC | This study |
CmR-F | ACCTTATGAAGTTTACTTCAAAAAAAGACTAAAAGAGAAGGGAGT | This study |
Sac-R | CCAAGCGAGCTCGATATCAA | This study |
Sac-F | TTGATATCGAGCTCGCTTGG | This study |
CmR-R | ATTATATTCTAGTTGATGAGTACTTCTTTTTCTCTTTAAGTTGAGGCTTAGCC | This study |
IR-R | AAAAAGAAGTACTCATCAACTAGAATATAATTTTGTTTCCACAC | This study |
P2 | ATTGCTGTGCGTCTGCAGCCA | This study |
UP-TF | AGACTAAAAGAGAAGGGAGTG | This study |
UP-TR | CGATTAAACTTGTTCACCCTTC | This study |
DO-F | CTCTCAAGTGTATATTCAGTATGGG | This study |
DO-R | CTCTTTAAGTTGAGGCTTAGCC | This study |
CTItestU | CGATGATAACAGCGTGGTGATC | This study |
CTItestD | TTGCCGATGCTTGATAGTATGC | This study |
MIS-F | CAATCAGTGGAGCGAAGTTG | This study |
MIS-R | CTGTTTTGTGCGTTCGTTGC | This study |
aRestriction sites are underlined.