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. 2019 Jan 22;10:15. doi: 10.3389/fmicb.2019.00015

Table 2.

Primers used for plasmid construction.

Primer Sequence ( 5′ → 3′)a Reference
TUtestF-BglII TACGCAGATCTACTACTGTGGCTAAGCCTCAAC This study
TUtestR-XhoI TACGCCTCGAGACGGAGAGTAACAACACGATGC This study
R6K-BglII TACGCAGATCTCCATGTCAGCCGTTAAGTGT This study
R6K-XhoI TACGCCTCGAGGTTGATCGGCACGTAAGAGG This study
R6K-BamHI TACGCGGATCCGTTGATCGGCACGTAAGAGG This study
R6K-EcoRI TACGCGAATTCCCATGTCAGCCGTTAAGTGT This study
P1 GGCTGCAGACGCACAGCA This study
IR-F TTTTTTGAAGTAAACTTCATAAGGTGTTTTCCAACC This study
CmR-F ACCTTATGAAGTTTACTTCAAAAAAAGACTAAAAGAGAAGGGAGT This study
Sac-R CCAAGCGAGCTCGATATCAA This study
Sac-F TTGATATCGAGCTCGCTTGG This study
CmR-R ATTATATTCTAGTTGATGAGTACTTCTTTTTCTCTTTAAGTTGAGGCTTAGCC This study
IR-R AAAAAGAAGTACTCATCAACTAGAATATAATTTTGTTTCCACAC This study
P2 ATTGCTGTGCGTCTGCAGCCA This study
UP-TF AGACTAAAAGAGAAGGGAGTG This study
UP-TR CGATTAAACTTGTTCACCCTTC This study
DO-F CTCTCAAGTGTATATTCAGTATGGG This study
DO-R CTCTTTAAGTTGAGGCTTAGCC This study
CTItestU CGATGATAACAGCGTGGTGATC This study
CTItestD TTGCCGATGCTTGATAGTATGC This study
MIS-F CAATCAGTGGAGCGAAGTTG This study
MIS-R CTGTTTTGTGCGTTCGTTGC This study

aRestriction sites are underlined.