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. 2019 Jan 22;10:12. doi: 10.3389/fphar.2019.00012

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Copyright © 2019 Hou, Cui, Zhou, Liu, Zhang, Ding, Nie and Ji.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Influenza virus decreased the transcriptional expression of ENaC. The mRNA samples were isolated from MTECs infected with influenza virus (Flu) for 24 h. Relative levels of mRNA were calculated as α- or β- or γ-ENaC/GAPDH ratios. (A–C) Quantitive-real-time PCR results for α-, β-, and γ-ENaC mRNAs. ^∗∗P < 0.01, ^∗∗∗P < 0.001, compared with control (Con), n = 4–5. Data was presented as mean ± SE Student’s t-test was used to analyze the difference of the means for significance in (A,B), and Mann–Whitney test was used to analyze the difference of the means for significance in (C).