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. 2019 Jan 22;10:2. doi: 10.3389/fimmu.2019.00002

Figure 1.

Figure 1

Generation of HSV-1 d106S-MelanA. (A) Agarose gel image of full-length MelanA amplified from expression plasmid pcDNA3(+) MART-1 (29) using XbaI/NheI-containing primers. (B) Light microscopic images of uninfected E11 cells (left) and cytopathic effects induced in these cells 56 h post HSV-1 d106S infection (right). (C) EcoRI digestion of HSV-1 d106S DNA obtained from viral nucleocapsids showing distinct bands as evidence of DNA integrity. (D) Overlay of phase contrast and immunofluorescence microscopy of E11 cells harboring fluorescing (upper part) and non-fluorescing (lower part) viral plaques, representing HSV-1 d106S and HSV-1 d106S-MelanA, respectively, after cotransfection of the linearized transfer plasmid pd27B-MelanA and HSV-1 d106S DNA. Light and immunofluorescence microscopy were taken using the DMI 6000B inverted microscope (20 × magnification).