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. 2019 Jan 22;10:2. doi: 10.3389/fimmu.2019.00002

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Copyright © 2019 Boscheinen, Thomann, Knipe, DeLuca, Schuler-Thurner, Gross, Dörrie, Schaft, Bach, Rohrhofer, Werner-Klein, Schmidt and Schuster.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of HSV-1 d106S-MelanA. MelanA expression in E11 cells infected with HSV-1 d106S and two non-fluorescent viral clones (MOI 10), which were obtained after cotransfection using HSV-1 d106S DNA and transfer plasmid pd27B-MelanA, as evident from (A) PCR, (B) Western blot, and (C) flow cytometry. Controls were (A) housekeeping gene ß-glucuronidase (GUS) and (B) ß-actin protein. (D) Expression of GFP and MelanA in Vero cells infected with HSV-1 d106S and two non-fluorescent HSV-1 d106S-MelanA clones, analyzed using immunofluorescence microscopy (upper panel) and confocal imaging (lower panel). The immunofluorescence and confocal images were taken using the DMI 6000B inverted microscope (20 × magnification) and the TCS SP5 laser scanning microscope (40 × magnification, 2.5 × zoom), respectively. Scale bars represent 50 and 10 μm in immunofluorescence and confocal images, respectively.