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. 2018 Nov 29;38(2):195–209. doi: 10.1007/s00299-018-2362-x

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — The effectiveness of microspore embryogenesis (mean number of embryo-like structures produced per spike of the donor plant ± SE) in isolated microspore cultures of winter triticale as the effect of various pre-treatments of tillers. The mean from at least three biological replications (isolations) ± SE. Axis Y is in the log scale. The asterisk symbol ‘*’ indicates values that are statistically different (p ≤ 0.05) from control (LT pre-treatment of tillers). LT low temperature pre-treatment of tillers (21 days at 4 °C); LT + 3–8d GSH low temperature pre-treatment of tillers (21 days at 4 °C) combined with short GSH pre-treatment (0.3 mM GSH applied 3–8 days before microspore isolation); LT + GSH low temperature pre-treatment of tillers (21 days at 4 °C) combined with long GSH pre-treatment (0.3 mM GSH applied 21 days before microspore isolation)