Fig. 3.

The effect of low temperature (LT) and glutathione (GSH) treatments on the viability of isolated microspores of DH lines of triticale (× Triticosecale Wittm.) with embryogenic potential. Viability of microspores isolated from tillers: non-treated (NT) (a, d, g, j, m), treated with low temperature (LT) at 4 °C for 21 days (b, e, h, k, n), and after combined treatment with LT and reduced glutathione (LT + GSH) at 4 °C for 21 days (c, f, i, l, o). a–o Intensive green (FITC) caused by FDA staining demonstrates vital microspores. Bar 50 µm. The sources of variance for microspore viability were as follows: five DH lines, three treatments, and interaction between DH line and treatment. ***Significant at p ≤ 0.001; ns not significant