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. 2018 Nov 29;38(2):195–209. doi: 10.1007/s00299-018-2362-x

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — The effect of low temperature (LT) and glutathione (LT + GSH) treatments on embryo-like structures (ELSs) production in DH lines of triticale (× Triticosecale Wittm.) with different embryogenic potential. a–h Isolated microspores of the recalcitrant DH19 line after LT (a–d) and glutathione (LT + GSH; e–h) treatments. i–p Isolated microspores of the responsive DH28 line after LT (i–l) and glutathione (LT + GSH; m–p) treatments. a, e, i, m Microspore suspension on the isolation day (0d). b, f, j, n Multicellular structures after 7 days (7d) of in vitro culture. c, g, k, o Multicellular structures and ELSs after 14 days (14d) of in vitro culture. d, h, l, p ELSs after 21 days (21d) of in vitro culture. Bar 50 µm