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. 2018 Nov 29;38(2):195–209. doi: 10.1007/s00299-018-2362-x

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 5 — The effectiveness of ELS formation and total plant regeneration as the effect of stress pre-treatment applied to tillers of responsive and recalcitrant DH lines of triticale (a–h). Induction phase: embryo-like structures after 6 weeks of in vitro culture (a, c, e, g). Regeneration phase: green and albino plantlets derived from ELS on solid medium (b, d, f, h). Numeric data indicate the mean number of ELS (a, c, e, g) and the mean total number of regenerants (b, d, f, h) per spike of the donor plant ± SE. LT—low temperature pre-treatment of tillers (21 days at 4 °C); LT + GSH—low temperature pre-treatment of tillers (21 days at 4 °C) combined with long GSH pre-treatment (0.3 mM GSH applied 21 days before microspore isolation)