Table 2.
The frequency of embryogenic microspores1 in microspore suspension of DH lines of triticale on the day of isolation as the effect of various tiller pre-treatments (experiment 2)
| DH line | LT | LT + GSH | LT + 4dGSH |
|---|---|---|---|
| DH119 | 7.4 ± 1.3bcd | 3.6 ± 1.0d | – |
| DH19 | 7.5 ± 2.5bcd | 2.2 ± 2.2d | 20.8 ± 4.6a |
| DH47 | 16.6 ± 7.2abc | 6.6 ± 2.7cd | 13.9 ± 2.4abc |
| DH28 | 18.3 ± 2.2ab | 17.3 ± 3.5abc | – |
| DH18 | 21.1 ± 9.9a | 13.1 ± 1.7abcd | – |
| Source of variation | Mean squares/p |
|---|---|
| DH line | 532.186/*** |
| Treatment | 515.555/* |
| DH line × treatment | 42.882/ns |
The mean from at least three biological replications (isolations) ± SE. Data marked with the same letter do not differ according to the Duncan test (p ≤ 0.05). The sources of variance for embryogenic potential were as follows: five DH lines, three treatments, and interaction between DH line and treatment
LT low temperature pre-treatment of tillers (21 days at 4 °C); LT + 3–8d GSH low temperature pre-treatment of tillers (21 days at 4 °C) combined with short GSH pre-treatment (0.3 mM GSH applied 3–8 days before microspore isolation); LT + GSH low temperature pre-treatment of tillers (21 days at 4 °C) combined with long GSH pre-treatment (0.3 mM GSH applied 21 days before microspore isolation)
*, ***Significant at p ≤ 0.05, 0.001, respectively; ns not significant
1The percentage of star-like structures (SLS) and microspores undergoing the first symmetrical nucleus division
‘–’ 4-day GSH pre-treatment was not applied