Fig. 5. C3^−/−C3^C163A, C3^−/−C3 ∆10, C3^−/−C3 ∆19, and C3^−/−C3 D9A are unable to remove the prodomain.

a Diagram showing the resulting molecular weight bands, detected from an anti-caspase-3 antibody that binds to the p20 domain, resulting from cleavage of the interdomain linker and the prodomain. b Cell lines were serum starved and lysates collected at the times indicated. Western blot analysis was conducted to determine procaspase-3 cleavage. c Samples serum starved for 24 h were run on the same gel for direct comparison. Actin was used as a loading control