Fig. 6. C3 D9E is inefficiently cleaved resulting in decreased caspase activation and cell death.

a Expression of cells stably expressing C3 and C3 D9E. b A caspase-3 activity assay was used to determine the ability to cleave a DEVD-chromphore substrate. c Cell death was determined using Annexin V/PI staining and flow cytometry. d Cells were serum starved for 24 h and the cleavage pattern of caspase-3 was determined using western blot analysis. Actin was used as a loading control. Data are presented as mean ± SEM of at least three independent experiments. *p > 0.05, **p > 0.01, ***p > 0.001