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. 2019 Jan 28;9:789. doi: 10.1038/s41598-018-37283-2

Figure 3.

Figure 3

(A) GLUT1 relative expression in MKN-28 cells incubated with 20 nM or 30 nM gold nanoparticles functionalized with 30% PEG and anti-GLUT1 (AuNP@PEG@anti-GLUT1) for 9 h, 12 h and 24 h at 37 °C, 5% (v/v) CO2 and 99% (v/v) relative humidity. (B) GLUT3 relative expression in MKN-28 cells incubated with 20 nM or 30 nM gold nanoparticles functionalized with 30% PEG and anti-GLUT3 (AuNP@PEG@anti-GLUT3) for 9 h, 12 h and 24 h at 37 °C, 5% (v/v) CO2 and 99% (v/v) relative humidity. Gene expression variation is calculated via 2−∆∆Ct, using as reference GAPDH gene. Error bars represents SEM of at least three independent experiments. *p-value < 0.05 relative to relative expression at 9 h incubation of corresponding sample.