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. 2019 Jan 28;9:789. doi: 10.1038/s41598-018-37283-2

Figure 6.

Figure 6

(A) Western Blot analysis of GLUT1 and β-actin proteins. (B) Western Blot analysis of GLUT3 and β-actin proteins. Represented Western Blots correspond to 10 μg total protein of MKN-28 cells grown on transwell plates and incubated for 24 h with fresh RPMI medium supplemented with 5.5 mM fructose and 0.75 nM AuNP@PEG1, 0.63 nM AuNP@PEG3, 1.38 nM AuNP@PEG1 + 3, 30 nM AuNP@GLUT1, 20 nM AuNP@GLUT3, or a mixture of AuNP@GLUT1 + 3. After this period of time, cells were incubated for an additional 24 h with fresh medium supplemented as previously. (C) GLUT1 relative intensity values normalized to corresponding intensity of β-actin protein and to the corresponding AuNP@PEG control sample. (D) GLUT3 relative intensity values normalized to corresponding intensity of β-actin protein and to the corresponding AuNP@PEG control sample. Error bars represents SEM of at least three independent experiments. The grey line on the border of blots represent the place where images were cropped. Full length blot images can be found on Supplementary Fig. S3. *p-value < 0.5, **p-value < 0.005, ***p-value < 0.0005.