Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 28;10:453. doi: 10.1038/s41467-019-08315-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — Deletion of Smad4 specifically in arterial endothelial cells does not affect vascular patterning or early embryonic development. a, b Representative pan-endothelial Tie2:Cre;Smad4^fl/+ and Tie2:Cre;Smad4^fl/fl whole-mount images (a) and transverse sections (b) from E10.5 embryos also transgenic for the Cre-reporter Rosa26R:LacZ. The cardinal vein (cv) cannot be identified in the Smad4^fl/fl embryo, although the dorsal aorta (da) can be seen in both. Grey scale bars are 500 μm, black scale bars are 100 μm. c, d Representative arterial-specific Dll4in3:Cre;Smad4^fl/+ and Dll4in3:Cre;Smad4^fl/fl whole-mount images (c) and transverse sections (d) from E10.5 embryos also transgenic for the Cre-reporter Rosa26R:LacZ. Arterial-specific deletion of Smad4 had no effect on vasculature development at E10.5. Grey scale bars are 500 μm, black scale bars are 100 μm. e Observed frequency of Smad4^fl/fl;Dll4in3:Cre embryos from E9.5 to P5. Only two sick (*) P5 Smad4^fl/fl;Dll4in3:Cre animals were recovered. ^EC indicates Tie2:Cre-mediated deletion, ^ART indicates Dll4in3:Cre-mediated deletion, da = dorsal aorta, nt = neural tube, cv = cardinal vein, ica = inner cerebral artery, isa = intersomitic arteries. See also Supplementary Figure 3