Table 1.

Sequence of primers used for genotyping and real-time PCR.

Gene	Application	Forward primer sequence (5′ to 3′)	Reverse primer sequence (5′ to 3′)	Product size (base pairs)
LacZ	Genotyping	AATGGTCTGCTGCTGCTGAAC	GGCTTCATCCACCACATACAG	225
rtTA	Genotyping	TTTCGATCTGGACATGTTGGG	GGCATCGGTAAACATCTGCTC	184
ApoE-WT	Genotyping	GCCTAGCCGAGGGAGAGCCG	TGTGACTTGGGAGCTCTGCAGC	155
ApoE-KO	Genotyping	GCCTAGCCGAGGGAGAGCCG	GCCGCCCCGACTGCATCT	245
18 S	Real-time PCR	GATGCTCTTAGCTGAGTGT	GCTCTGGTCCGTCTTG	233
DNT5	Real-time PCR	CTCTTCAGTCAGCTGCAGCG	TTCTCCGTCTACCGTCAGGG	360
AdipoQ	Real-time PCR	GTATCGCTCAGCGTTC	CGTTGACGTTATCTGCAT	362
Lep	Real-time PCR	CATTTCACACACGCAGT	GGAGGTCTCGGAGATTC	191
Tnfa	Real-time PCR	CCACCACGCTCTTCTGTCTAC	AGGGTCTGGGCCATAGAACT	103
Il6	Real-time PCR	TGATGCACTTGCAGAAAACA	ACCAGAGGAAATTTTCAATAGGC	109
Il1b	Real-time PCR	GCAACTGTTCCTGAACTCAACT	ATCTTTTGGGGTCCGTCAACT	89
Ccl2/Mcp1	Real-time PCR	TGAGTAGGCTGGAGAGCTACAAG	TGTATGTCTGGACCCATTCCTTC	126
Ccl3/Mip1a	Real-time PCR	TGAAACCAGCAGCCTTTGCTC	AGGCATTCAGTTCCAGGTCAGTG	125
Ccl5/Rantes	Real-time PCR	CTCACCATATGGCTCGGACA	ACAAACACGACTGCAAGATTGG	126
Ccl7/Mcp3	Real-time PCR	GCTGCTTTCAGCATCCAAGTG	CCAGGGACACCGACTACTG	135
Srebp1c	Real-time PCR	GGAGCCATGGATTGCACATT	CACTGTCTTGGTTGTTGATGAGCTG	58
Acaca	Real-time PCR	GCCTCTTCCTGACAAACGAG	TGACTGCCGAAACATCTCTG	239
Fasn	Real-time PCR	GCTGCGGAAACTTCAGGAAAT	AGAGACGTGTCACTCCTGGACTT	83
Scd1	Real-time PCR	CCCTGCGGATCTTCCTTATC	TGTGTTTCTGAGAACTTGTGGTG	103