Fig. 3. Caspase-3 activities induced by sigma-2 ligands.

Control, TMEM97 KO, PGRMC1 KO, or double KO cells were treated with increasing concentrations of sigma-2 ligands for 24 h. Caspase-3 activation was then determined by Caspase-Glo 3/7 Assay. a, b Representative dose–response curves of caspase-3 activity for siramesine (a) and SW43 (b). c EC₅₀ values of caspase-3 activation of sigma-2 ligands were represented as bar graphs. EC₅₀ values were reported as mean ± SD in at least two independent experiments performed in triplicates. No significant difference existed for EC₅₀ values of sigma-2 ligands in TMEM97 KO, PGRMC1 KO, or double KO cells vs the control cells by two-way Anova analysis with one exception, i.e., there was a significant difference (asterisk) of EC₅₀ of siramesine in double KO cells vs control cells