Figure 20.

In vitro direct cutlure of nHA_400, mHA_400 and Mg plates with BMSCs for 24 hours and the resulted BMSC morphology, cell adhesion density, and post-culure media analyses. Mg plate samples had an initial dimension of 7.5 mm in diameter and 1 mm in height. (a) Flourensence images of BMSCs that were under direct contact or indirect contract conditions with nHA_400, mHA_400 and Mg plates after 24 hours of direct culture. (b) BMSC adhesion densities after 24 hours of culture were quantified based on the fluoresence images using ImageJ. (c) The pH of media after cultured with the respective Mg plate samples. (d) Mg²⁺ ion concentrations in the media after 24 hours of BMSC culture. (e) Ca²⁺ ion concentrations in the media after 24 hours of BMSC cutlure. Data are mean ± standard deviation (N = 3); *p < 0.05, ***p < 0.001, ****p < 0.0001.