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. 2019 Jan 28;5:57. doi: 10.1038/s41420-019-0138-x

Fig. 4. An osteogenic-like process is activated in HK-2 cells grown in osteogenic conditions.

Fig. 4

a qRT/PCR of osteogenesis-related genes: Runx2, ALP, osteonectin (ON) and osteopontin (OP). For ON and OP, the results are expressed as the ratio of ON to OP, indicating the balance between pro- and anti-osteogenic factors. Data are presented as the mean ± SD of three separate experiments. *p < 0.05; ***p < 0.005. b Runx2 protein quantification by in-cell Western assay. Data are presented as the mean ± SD of three separate experiments. ***p < .0.005. c Immunolabeling of Runx2, osteonectin and osteopontin, and ALP activity in HK-2 cells in osteogenic and standard conditions. Immunostaining images of cells in NH OsteoDiff medium: Bar = 50 µm. Inserts show details of immunostaining. Bar = 25 µm. Immunostaining images of cells in DMEM/HAM'S F12: Bar= 25 μm. ALP images: Bar = 25 µm