Table 1.
Primary antibodies and dilutions used for In-Cell Western assays
| Target | Clone | Host | Manufacturer | Code | Dilution |
|---|---|---|---|---|---|
| Cleaved Caspase-9 (Asp330) | D2D4 | rabbit | Cell Signaling Technology | CST-7237 | 1:1000 |
| Cleaved Caspase-3 (Asp175) | 5A1E | rabbit | Cell Signaling Technology | CST-9664 | 1:1000 |
| Cleaved Caspase-6 (Asp162) | — | rabbit | Cell Signaling Technology | CST-9761 | 1:1000 |
| Cleaved Caspase-7 (Asp198) | D6H1 | rabbit | Cell Signaling Technology | CST-9761 | 1:1000 |
| Cleaved PARP (Asp214) | D64E10 | rabbit | Cell Signaling Technology | CST-5625 | 1:1000 |
| Runx2/CBFA1 | — | rabbit | Novus Biologicals | NBP1-77461 | 1:50 |
| β-Tubulin | H235 | rabbit | Santa Cruz Biotechnology | sc-9104 | 1:50 |