Table 2.
Primer sequences used in the qRT/PCR analyses
| Primers | Nucleotide sequence (5′–3′) | T annealing (°C) | NCBI reference sequence | Efficiency (%) |
|---|---|---|---|---|
| GAPDH Fw GAPDH Rev |
GAAGGTGAAGGTCGGAGT TGGCAACAATATCCACTTTACCA |
60 | NM_17851.1 | 99.0 |
| HPRT1 Fw HPRT1 Rev |
CCTGGCGTCGTCATTAGTGA TCTCGAGCAAGACGTTCAGT |
60 | NM_000194.2 | 97.8 |
| Osteonectin Fw Osteonectin Rev |
CCTGGATCTTCTTTCTCCTTTGC ATCAGGCAGGGCTTCTTGCT |
60 | NM_001309443.1 | 97.9 |
| Osteopontin Fw Osteopontin Rev |
CGAGACCTGACATCCAGTACC GATGGCCTTGTATGCACCATTC |
62 | NM_001251830.1 | 96.2 |
| Runx2 Fw Runx2 Rev |
CATTTCAGATGATGACACTGCC GGATGAAATGCTTGGGAACTG |
62 | NM_001024630.3 | 95.2 |
| ALP Fw ALP Rev |
GGCAACTCTATCTTTGGTCTG GTTGTTGTGAGCATAGTCCA |
60 | NM_000478.4 | 95.0 |
| BAX Fw BAX Rev |
GCCGTGGACACAGACTCC AAGTAGAAAAGGGCGAAACC |
60 | NM_001291428.1 | 91.2 |
| BCL-2 Fw BCL-2 Rev |
TCATGTGTGTGGAGAGCGTCAA CAGCCAGGAGAAATCAAACAGAGG |
60 | NM_000633.2 | 88.9 |