Fig. 5. High cholesterol diet (HCD) induced white matter inflammation selectively in TGF mice, a response significantly reduced by simvastatin (SV).

Cresyl-violet stained brain sections show the targeted areas (dashed line) of the corpus callosum (cc, a) and internal capsule (IC, b) used for quantitative analysis (top right panels, respectively). In TGF mice, galectin-3 (Gal-3)-immunofluorescent (green, Cy2) cell number (a) or surface area (b) was dramatically increased by HCD (arrows, right panels, respectively), an increase that was significantly reduced by SV treatment in both regions, and not seen in HCD-treated WT mice. Cpu caudate putamen, cg cingulate cortex, LV lateral ventricle, fi fibria. Bars: 200 μm (cc) and 300 μm (IC), *p < 0.05; ***p < 0.001