Figure 3.

(A) Western blots of GFP-tagged G12V-KRAS from cell extracts without a solubilizing agent (PBS) and after treatment with DDM or SMA (see text). (B) Western analysis, using an anti-HA antibody, of different volumes of SMA-solubilized cell extract pellet (lanes 1–2) or supernatant (lanes 3–4) is shown, with β-actin used as loading control. (C) FRET efficiency (E_A) histogram fitted to three Gaussians, indicating three distinct conformational states, with high FRET states in blue, mid-FRET states in green, and low FRET states in red. A representative E_A trajectory indicating fast transitions between states is shown as inset, with the actual signal in red and state transitions highlighted in black. To see this figure in color, go online.