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. 2018 Dec 19;116(2):227–238. doi: 10.1016/j.bpj.2018.12.010

Figure 1.

Figure 1

The effect of N-terminus-bound fluorophores on the fibrillation kinetics and fibril morphology of Aβ40. (A) Fluorescence labeling of the unstructured N-terminus of Aβ40 is expected to have the least effect on structure formation (presentation is based upon Protein Data Bank (PDB) structure 2LFM (55) and plotted by the NGL viewer (56)). Inset: amino acid sequence of Aβ40 with secondary structure prediction using RaptorX (57). (B) The time-dependent ThT fluorescence of Aβ40wt without (black) and with 5% of Atto488-Aβ40 (blue) or HL647-Aβ40 (red). The time axis is normalized to the half time of Aβ40wt. (C–H) Representative TEM (C–E) and AFM (F–H) images of the fibrils after 24 h of incubation. The scale bar represents 500 and 200 nm for TEM and AFM, respectively. To see this figure in color, go online.