Table 1.

Characteristics of Labeled Aβ40 Peptides and Their Oligomeric Aggregates

Peptide	Oligomer Size Distribution (DOL 5%)		Fluorescence Lifetime		Anisotropy Decay Characteristics
	μ1	${\mu }_2^{1/2}$	${\tau }_F^{mon}$/ns	${\tau }_F^{olig}$/ns	S2	${\tau }_r^f$/ns	$D_{\perp }$/ns−1
Atto488-Aβ40	2.7 ± 0.2	4.7 ± 0.4	3.2 ± 0.1	3.1 ± 0.1	0.12 ± 0.01	0.51 ± 0.02	0.288 ± 0.005
HL488-Aβ40	4.1 ± 0.2	6.3 ± 0.4	3.2 ± 0.1	3.1 ± 0.1	0.28 ± 0.01	0.68 ± 0.03	0.168 ± 0.013
Atto655-Aβ40	7.4 ± 0.2	8.8 ± 0.4	1.9 ± 0.1	2.4 ± 0.1	0.68 ± 0.03	0.54 ± 0.09	0.081 ± 0.023
HL647-Aβ40	10.6 ± 0.2	14.9 ± 0.4	2.0 ± 0.1	2.8 ± 0.1	–	1.77 ± 0.02a	0.094 ± 0.003a

Shown are the first moments, ${\mu }_1={\overline{N}}_{phot,rel}$, and the roots of the second moments, ${\mu }_2^{1/2}={\left({\overline{N^2}}_{phot,rel}\right)}^{1/2}$, of the oligomer size distributions, the average fluorescence lifetimes, and parameters describing the anisotropy decays. The initial monomer concentration was 40 μM.

^aFor HL647-Aβ40, the parameters refer to a single exponential decay.