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Primer sequences in PCR assay
| Gene name | Direction | Sequence (5′–3′) | Length (bp) | TM (°C) |
|---|---|---|---|---|
| GAPDH | Forward | GGAGCGAGATCCCTCCAAAAT | 197 | 61 |
| Reverse | GGCTGTTGTCATACTTCTCATGG | |||
| RP11-269F21.2 | Forward | AGGTTCCAAACCAGGAGCT | 300 | 60 |
| Reverse | CCCTTCTACCTTGGCAGTGA | |||
| DIAPH2-AS1 | Forward | GGATCTTGCATTGGAGGAGA | 166 | 60.5 |
| Reverse | ATGGTGGCACGTGTCTGTA | |||
| RP11-445K13.2 | Forward | GCCCCAGAGAGATATGTCCA | 188 | 60 |
| Reverse | TGAAGGCACTGCAATCATGT | |||
