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. 2018 Dec 29;28(1):28–43. doi: 10.1089/scd.2018.0058

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© Shota Fujii et al. 2018; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are cited.

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FIG. 2. — Expression of HLA class I, class II, and costimulatory molecules on iPSC-NCCs. (A) For the flow cytometry analysis, HLA class I expression of iPSC-NCCs is lower compared with the iPSCs. Expression of β2-microglobulin of the iPSC-NCCs is lower compared with iPSCs. Expression of HLA class II is not detected in either of the cells. MFI, mean fluorescence intensity. (B) MFI of HLA class I and β2-microglobulin was also examined. Data are the mean ± SD of three experiments. * P < 0.01, comparing two groups. (C) Expressions of CD80 (B7-1), CD86 (B7-2), CD274 (PD-L1), and CD273 (PD-L2) failed to be detected in the iPSCs and iPSC-NCCs. Red histogram; isotype control.