FIG. 5.

Ability of iPSC-NCCs to suppress T cells through cell contact molecules. (A) iPSC-NCCs were first cultured in 12-well plates. Transwell cell inserts were placed in these wells, and each transwell contained PBMCs plus anti-CD3 and anti-CD28 antibodies to block cell-to-cell contact between the NCCs and CD3 positive T cells in PBMCs. The numbers in the FACS histograms indicate CD3-Ki-67 double-positive cells. These data are representative of three experiments. (B) Percentages of the proliferating T cells (double-positive cells in A) were also examined. Data are the mean ± SD of three experiments. *P < 0.05, compared to the two groups. NS, not significant.