. 2019 Jan 25;39(1):BSR20181733. doi: 10.1042/BSR20181733

Table 5.

Digestive α-amylase activity (U/mg protein) in male Holstein calves fed milk and a starter supplemented with leucine and phenylalanine

Sampling Site (%)	Treatments¹				SEM	P-value
	C	P	L	PL		P	L	P × L
0	2.42^b	2.15^bc	2.47^b	3.53^ab	0.334	0.560	0.305	0.339
20	1.44^b	3.13^bc	1.64^b	2.02^b	0.313	0.477	0.117	0.312
40	1.44^b	1.03^c	1.39^b	2.03^b	0.236	0.815	0.331	0.279
60	3.57^ab	3.12^bc	4.11^b	1.90^b	0.655	0.327	0.800	0.515
80	4.52^ab	8.76^b	6.49^a	8.13^a	1.34	0.805	0.288	0.633
100	7.64^a	15.08^a	9.23^a	7.13^a	1.55	0.403	0.321	0.144
Site effect					P<0.001
Interaction between Site and Treatment					P=0.054

C, control; P, phenylalanine; L, leucine; PL, leucine plus phenylalanine. Different lowercase letters in a column indicate statistically significant differences (P<0.05). The total length of the small intestine from the distal region of the pylorus to the ileal-cecal junction was defined as 100%. The digesta samples at the 0, 20, 40, 60, 80, and 100% relative sites were collected to determine enzyme activities.