Table 4.

Association Between TILs and Immunocheckpoint Markers

a
	CD4			CD8			FOXP3
	Negative	Positive	P	Negative	Positive	P	Negative	Positive	P
	n (%)	n (%)		n (%)	n (%)		n (%)	n (%)
CTLA4 in TILs
Negative	54 (70.1)	38 (48.1)	.005	59 (71.9)	36 (43.4)	.0002	59 (80.8)	35 (37.6)	<.0001
Positive	23 (29.9)	41 (51.9)		23 (28.1)	47 (56.6)		14 (19.2)	58 (62.4)
PD-1 in TILs
Negative	60 (85.7)	45 (59.2)	.0004	63 (85.1)	46 (59.7)	.0005	47 (78.3)	60 (67.4)	.148
Positive	10 (14.3)	31 (40.8)		11 (14.9)	31 (40.3)		13 (21.7)	29 (32.6)
PD-L1 in TILs
Negative	72 (96)	45 (59.2)	<.0001	77 (96.2)	49 (60.5)	<.0001	68 (93.1)	59 (66.3)	<.0001
Positive	3 (4)	31 (40.8)		3 (3.8)	32 (39.5)		5 (6.9)	30 (33.7)
PD-L1 in tumor cells
Negative	75 (100)	70 (90.9)	.014	79 (98.7)	76 (92.7)	.117	71 (95.9)	85 (95.5)	.890
Positive	0 (0)	7 (9.1)		1 (1.3)	6 (7.3)		3 (4.1)	4 (4.5)

b
	sCD4			iCD4			sCD8			iCD8
	Negative	Positive	P	Negative	Positive	P	Negative	Positive	P	Negative	Positive	P
	n (%)	n (%)		n (%)	n (%)		n (%)	n (%)		n (%)	n (%)
CTLA4 in TILs
Negative	51 (66.2)	41 (51.9)	.070	53 (66.2)	40 (52.6)	.084	53 (64.6)	42 (50.6)	.069	49 (62.8)	46 (52.9)	.198
Positive	26 (33.8)	38 (48.1)		27 (33.8)	36 (47.4)		29 (35.4)	41 (49.4)		29 (37.2)	41 (47.1)
PD-1 in TILs
Negative	60 (84.5)	45 (60)	.001	60 (76.9)	45 (67.2)	.191	63 (84)	46 (60.5)	.001	61 (85.9)	48 (60)	.0004
Positive	11 (15.5)	30 (40)		18 (23.1)	22 (32.8)		12 (16)	30 (39.5)		10 (14.1)	32 (40)
PD-L1 in TILs
Negative	62 (84.9)	55 (70.5)	.035	71 (91)	46 (63)	<.0001	67 (83.7)	59 (72.8)	.094	70 (92.1)	56 (65.9)	<.0001
Positive	11 (15.1)	23 (29.5)		7 (9)	27 (37)		13 (16.3)	22 (27.2)		6 (7.9)	29 (34.1)
PD-L1 in tumor cells
Negative	72 (98.6)	73 (92.4)	.118	79 (100)	66 (90.4)	.005	77 (96.2)	78 (95.1)	.725	75 (98.7)	80 (93)	.122
Positive	1 (1.4)	6 (7.6)		0 (0)	7 (9.6)		3 (3.8)	4 (4.9)		1 (1.3)	6 (7)

s = stromal.

i = intratumoral.