Table II.
Antibodies utilized.
| Antibody | Conjugate | Clone | Cat. no. | Supplier | Dilution | Stock concentration, µg/ml |
|---|---|---|---|---|---|---|
| CD45 | APC-H7 | 2D1 | 560178 | BD Biosciences | 1:50 | 200 |
| CD3 | FITC | UCHT1 | 5553332 | BD Biosciences | 1:25 | 12.5 |
| CD14 | FITC | TüK4 | MHCD1401 | Thermo Fisher Scientific, Inc. | 1:50 | Not available |
| CD56 | FITC | NCAM16.2 | 345811 | BD Biosciences | 1:50 | 6 |
| CD19 | Brilliant Blue515 (BB515) | HIB19 | 564456 | BD Biosciences | 1:25 | 100 |
| HLA-DR | PerCp-Cy5.5 | L243 | 307630 | BioLegend Inc., San Diego, CA, USA | 1:250 | 200 |
| CD11c | Brilliant Violet 421 (BV421) | B-ly6 | 562561 | BD Biosciences | 1:50 | 50 |
| CD1c | APC | L161 | 17-0015-42 | eBioscience; Thermo Fisher Scientific, Inc. | 1:50 | 6 |
| CD141 | Brilliant Violet 510 (BV510) | 1A4 | 563298 | BD Biosciences | 1:100 | 100 |
| CD123 | PE-Cy7 | 6H6 | 306010 | BioLegend Inc. | 1:100 | 200 |
| CD207 | PE | DCGM4 | IM3577 | Immunotech; Beckman Coulter, Inc., Brea, CA, USA | 1:50 | 25 |
| ChromPure mouse IgG, whole molecule | None | Not available | 015-000-003 | Jackson ImmunoResearch Laboratories, Inc., West Grove PA, USA | 1:19500 | 5900 |
APC, allophycocyanin; FITC, fluorescein; PE, phycoerythrin; HLA-DR, human leukocyte antigen-DR.