miR-16 regulates the expression of HMGA2. (A) Bioinformatic analysis revealed that there is a complementary region between seed region of miR-16 and 3′-UTR of HMGA2 gene. (B) Dual luciferase reporter assay was performed to detect the interaction between miR-16 and HMGA2. (C) The expression of miR-16 and HMGA2 was assessed using reverse transcription-quantitative polymerase chain reaction. (D) The expression of HMGA2 was assessed using western blot analysis. *P<0.05. miR, microRNA; HMGA2, high mobility group A2; hsa, homo sapiens; wt, wild-type; mut, mutant.