Figure 3.

Silencing of Ψ- and Ψ/m5C-Modified miRNA Switches Is Independent of the U or C Content

(A) Sequences of miR-126-3p, miR-21-5p, miR-145-5p, and miR-122-5p and their complementary target sites. ˆThe site of Argonaute cleavage. Red bases are complementary to the seed sequence. (B–E) Representative immunoblots (B and D) and densitometric quantification (C and E) of HEK293 cells transfected with the indicated miRNA mimics or vehicle control 24 h before transfection with the indicated miRNA switches. GFP and GAPDH expressions were measured after 24 h. Data represent the mean ± SEM, normalized against GAPDH and relative to vehicle control. *p < 0.05 versus miR-143 control. NTC, non-transfected control. Numbers above bars indicate the mean values.