Table 6.

In vitro chromosome aberration test in chinese hamster lung cells with OE extract from cactus stem

Treatment Schedulea	Dose (μg/mL)		Ratio of Aberrant Metaphaseb	Cell Countsc	Mean		RICC(%)d
6–18 (+S)	0		0	4792	4689	4741	100
	500		0	5024	4931	4978	108
	1500	P	0	4996	4774	4885	105
	3000	P	2	4405	4247	4326	86
	3500	P	2	4158	4107	4133	80
	4000	P	1	3528	3491	3510	59
	4500	TTP	1	3054	2971	3013	42
	5000	TTP	2	2796	2756	2776	34
	B[a]P 20		30	3066	3116	3091	45
6–18 (-S)	0		0	5899	5757	5828	100
	500		0	5525	5389	5457	91
	1500	P	1	5001	4962	4982	79
	1800	P	2	4243	4539	4391	65
	2000	P	0	3508	3445	3477	42
	3000	P	2	2942	2734	2838	26
	4NQO 0.4		18	3226	3148	3187	35
24–0 (-S)	0		0	5600	5444	5522	100
	500		1	5400	5479	5440	98
	1500	P	1	4971	4971	4971	85
	3000	P	1	4153	4072	4113	63
	4000	P	0	3863	3842	3853	56
	5000	TTP	0	3652	3670	3661	51
	4NQO 0.4		21	2853	2994	2924	31
Initial cell count		Cell countsc				Mean
		1754	1673	1872	1750	1762

^aTreatment time – Recovery time in the presence (+S) and absence (-S) of metabolic activation system

^bRatio of metaphase with chromosome aberrations. One culture/ dose was used

Gaps excludes, 100 metaphases/ culture were examined

^cAfter harvesting mitotic cells, each culture was trypsinized and suspended with 0.5 mL of 0.1% trypsin and 5 mL of culture medium

The cell suspensions of 0.4 mL per culture were diluted 50 times with 19.6 mL of Isoton sol. The cells in 0.5 mL Isoton sol. were counted twice/ culture using Coulter Counter model Z2

Actual number of cells per flask = Mean Count × 550

^dRelative Increase in cell Count = ((Cell count of treated flask – Initial cell count) / (Cell count of the negative control flask – initial cell count)) × 100 (%)

T: Turbidity at the end of the treatment

P: Precipitation at the end of the treatment

B[a]: benzo[a] pyrene, 4NQO: 4-Nitroquinoline-1-oxide