Fig. 11.

Knockdown of FOXO3a decreased SPRY2 protein stability while knockdown of SPRY2 enhanced β-catenin protein stability. a PANC-1 and SW1990 cells were transfected with FOXO3a specific siRNA or control siRNA for 48 h. Western blot was then used to determine the protein levels of SPRY2 in PDAC cells treated with CHX (100 μM) for indicated periods of time. b PANC-1 and SW1990 cells were transfected with SPRY2 specific siRNA or control siRNA for 48 h. Western blot was then used to determine the protein levels of β-catenin in PDAC cells treated with CHX (100 μM) for indicated periods of time. Quantified relative protein levels were calculated and half-lives were listed in legend. *P < 0.05, **P < 0.01