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. 2019 Jan 28;38:38. doi: 10.1186/s13046-019-1046-x

Table 1.

The sequences of primers for qRT-PCR

Gene Primer sequence
FOXO3a
 Forward 5′-CGGACAAACGGCTCACTCT-3′
 Reverse 5′-GGACCCGCATGAATCGACTAT-3′
SPRY2
 Forward 5′-CTCGGCCCAGAACGTGATT-3′
 Reverse 5′-GGCAAAAAGAGGGACATGACAC-3′
β-catenin
 Forward 5′-CATCTACACAGTTTGATGCTGCT-3′
 Reverse 5′-GCAGTTTTGTCAGTTCAGGGA-3′
TCF4
 Forward 5′-AGAAACGAATCAAAACAGCTCCT-3′
 Reverse 5′-CGGGATTTGTCTCGGAAACTT-3′
E-cad
 Forward 5′-CGAGAGCTACACGTTCACGG-3′
 Reverse 5′-GGGTGTCGAGGGAAAAATAGG-3′
VIM
 Forward 5′-GACGCCATCAACACCGAGTT-3′
 Reverse 5′-CTTTGTCGTTGGTTAGCTGGT-3′
β-actin
 Forward 5′-ATCACCATTGGCAATGAGCG-3′
 Reverse 5′-TTGAAGGTAGTTTCGTGGAT-3′